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Hello.
AWS blocks port 25, so communication is not possible even if it is allowed in the security group.
To be able to communicate, you need to remove the restriction by following the steps described in the document below.
https://repost.aws/knowledge-center/ec2-port-25-throttle
By the way, I have seen that even if you send a request, the request may not be allowed depending on the use case, so please be careful.
In some cases, it may be better to use Amazon SES or set up your email to be sent using an SMTP port other than port 25.
Nat Gateway allocated to EC2 instance Subnet - subnet-0b2660a2360e4a1a1