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Traffic should work. It shouldn't matter if the target is in Public or Private subnet, see below note from the documentation:
You can enable or disable the Availability Zones for your load balancer at any time. After you enable an Availability Zone, the load balancer starts routing requests to the registered targets in that Availability Zone.
Reference: https://docs.aws.amazon.com/elasticloadbalancing/latest/application/load-balancer-subnets.html
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As per aws "After you disable an Availability Zone, the targets in that Availability Zone remain registered with the load balancer, but the load balancer will not route requests to them."
A "public" subnet is simply a subnet with a route to an Internet Gateway. If the instance does not have a public IP assigned, it is not reachable from the internet.