Is it possible to create a signed URL for unique usage in AWS CloudFront ?

You are correct that CloudFront does not directly support generating single-use signed URLs with expiration times out of the box. However, you can achieve this functionality by leveraging other AWS services in combination with CloudFront. One common approach is to use Amazon S3 pre-signed URLs with CloudFront

Here's an overview of how you configure CloudFront and Amazon S3 for signed URLs and how CloudFront responds when a user uses a signed URL to request a file.

[1] https://docs.aws.amazon.com/AmazonCloudFront/latest/DeveloperGuide/private-content-signed-urls.html