After RDS OS patched in an offline operation RDS Database is not accepting connection from outside the VPC

After a offline operation RDS OS patch

Info:

• Status is available
• Connection attempts from outside the VPC is always receive timeout
• Connection Inside our VPC working
• Last time worked at Saturday 20:40 (outside attempts)
• RDS instance - 8.0.23
• Publicly accessible - yes

Note: Creating identical RDS instances in prior version to v8.0.23 Test RDS instances with same VPC configurations:

• v8.0.21 - working
• v8.0.23 - not working
• v8.0.28 - not working