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- You need routes in both VPC's subnets to reach each other via TGW
- You need to check Transit Gateway route tables associated with TGW attachments. If routes were not Propagated, you need to create Static routes
- (Optional) It is Better to refer to EKS Node SG ID in the INBOUND rule of RDS SG instead of the whole VPC CIDR. Anyway, your configuration of SG should work
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I was missing the route in one direction, even though I had it set for the other direction. So I just edited the route tables and made sure the subnets accept each other. Thank you so much!